Dear Colleagues, Two questions that I need a bit of help with. Firstly, measuring reticulocytes, I have used an thiazol orange method on human RBC's sometime ago. Is this still the best method of choice?. Also is it possible to class the retics into groups i.e. of differing staining intensity. Any comments from people performing retics routinely most welcome. Secondly, a student would like to perform some PI staining (looking at ploidy) in some lymphoma paraffin blocks. Removing the paraffin is not a problem but the digestion to a single cell suspension. I would appreciate any fool proof ! methods for digesting the tissue. Many thanks, Andy Macintyre. Department of Veterinary Pathology.
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