I would like to get some information from anyone who is successfully using the Modfit DNA Analysis program for analysis of DNA content, cell cycle, and especially apoptotic populations. We are having quite a bit of difficulty getting useful information from this program regarding apoptotic populations. We are using mostly PI staining of nuclei for analysis. We are also using cell lines, not clinical samples, so there is limited involvement of an aneuploid population. The main trouble is setting the markers so the data makes sense. We either see an apoptotic population under the G1 and S phases of the histogram or the apoptotic population is labeled as debris. We have no consistency in our data with regard to apoptosis. The manual is not helpful. If anyone out there can help, it sure would be appreciated. Best regards, Susan Zunino Dr. Susan J. Zunino Lehrstuhl fur Genetik University of Erlangen Staudtstr. 5 91058 Erlangen Germany Tele. 49 9131 8528784 FAX 49 9131 8528526 e-mail: szunino@biologie.uni-erlangen.de
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