Dear Flowers, We plan to start Ca++ determinations on a 488 laser. We will use the assay system to determine the capacity of synthetic peptides to "block" Ca++ flux induction by suboptimal amounts of stimulating CD21 antibody on bovine PBMC. It a long time since we did Ca++ experiments using Fluo-3, and, obviously, science has moved on. Could someone out there recommend a working protocol for Fluo-3 as well as Fluo 3 vs Fura Red Fluo pointing out the merits and demerits of each approach if possible? In our hands, Fluo-3 worked well. We intend to use the same reagents which have been stored at -20oC over the years. Would it be advisable to order fresh reagents or will the "old" work? Your experiences will be treasured. Thank you in advance. Joseph ******************************************************** Joseph Nthale Genetics of Disease Resistance (GDR) ILRI -International Livestock Research Institute, E-Mail: J.Nthale@cgiar.org Phone: +254-2-630743 Fax: +254-2-631499 P.O. Box 30709, Nairobi, Kenya > Visit the ILRI web site at http://www.cgiar.org/ilri ******************************************************** Future Harvest ILRI is one of 16 international agricultural research institutes supported by the Consultative Group on International Agricultural Research (CGIAR).
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