Do you pre-wash to remove serum immunoglobulin before staining for light chains? Howard Ratech, MD Albert Einstein College of Medicine Bronx, NY On Wed, 14 Jul 1999, Sebastian, J, Gelderbloem, Mr wrote: > > Dear Flowers > > I have a slight problem and I am not sure what the solution is. > > We use polyclonal Kappa and Lambda from BD for our chronic > leukaemia panel. We have been setting the samples up using whole > blood and for some or other reason the Light Chains are coming up > negative everytime. We only bitmap lymphoid cells. > > Is there anyone with some advice. > > Thanks > Sebastian >
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