Hello, I have been performing intracellular cytokine staining using Pharmingen antibodies (PE conjugates) to stain human monocytes for IL-6, TNF and IL-10 and was wondering what the sensitivty of the assay is in terms of the minimum amount (mass) of cytokine that can be detected. If anyone knows or can point me in the right direction I would greatly appreciate the help. Thanks, Suzanne Moss .
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