Re: GFP and temperature dependance?

From: Rachel M. Gerstein (Rachel.Gerstein@ummed.edu)
Date: Tue Jul 06 1999 - 16:16:14 EST


Hi Geoffrey,

The temperature can affect the stability of the GFP - how much depends on the
specific variant that you are using.  Body temperature is fine for GFP
stability for some variants, but the protein is expected to be more stable at
room temp or 4 degrees C.

Why do you suspect that it is the temperature that explains the difference in
the tail glowing (with UV light?) compared to bone marrow cells lack of GFP
detection by flow ?   Is the promoter you are using expected to give bone
marrow expression ? Have other people detected expression in the bone marrow
of these mice before ? Do you have positive controls for GFP detection by flow
?

You can post to the list or e-mail me privately.

Geoffrey Osborne wrote:

> Hi All,
>         I looked around but can't find any references to....
> Whether the level fluorescence of GFP is related to the temperature at
> which it is exposed to an appropriate light source?
> My query is related to the lack of detectable GFP signal by flow cytometry,
> from a GFP + transgenic mouse (the skin on the tail glows for example) bone
> marrow cells. If the temperature is important, then in the mammalian system
> the GFP may not be suitbale for the researchers needs.
>
> Thanks in advance,
> Geoff
> ======================================================================
> Geoffrey Osborne                           |  ____  __ o  Ahh!
> Flow Cytometry (FACS LAB)                  |  __   `\ <,_
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> Email: Geoff.Osborne@anu.edu.au            |                   |--|...
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