Hi All, I looked around but can't find any references to.... Whether the level fluorescence of GFP is related to the temperature at which it is exposed to an appropriate light source? My query is related to the lack of detectable GFP signal by flow cytometry, from a GFP + transgenic mouse (the skin on the tail glows for example) bone marrow cells. If the temperature is important, then in the mammalian system the GFP may not be suitbale for the researchers needs. Thanks in advance, Geoff ====================================================================== Geoffrey Osborne | ____ __ o Ahh! Flow Cytometry (FACS LAB) | __ `\ <,_ John Curtin School of Medical Research, | __ (*)/ (*) Australian National University, | ==============| CANBERRA, AUSTRALIA. | |--| Email: Geoff.Osborne@anu.edu.au | |--|... Phone: 61 2 6249 3694 | FAX: 61 2 6249 2595 | -----Surfing the Web?: Try http://jcsmr.anu.edu.au/facshome.html ======================================================================
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