Dual Laser sorting/FRET

From: Ann Atzberger (Ann.Atzberger@EMBL-Heidelberg.de)
Date: Wed Jul 07 1999 - 11:14:03 EST 

Hallo Flowbies, (I hope that's not a rude word)

2 Questions:FACS Vantage

1. When sorting with dual laser does one calculate the drop delay from the
2nd laser spot or from the first?. I've been using the 2nd and sorting
seems to be o.k (haven't checked recovery) but someone else I know
calculates from the 1st.

2. When doing FRET analysis should the laser with the lower wavelength
always be in position 1?

Haven't been able to find anything regarding the above anywhere.


slainte

Ann
EMBL
Heidelberg

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