> Does anyone knows what concentration of PMA has to be used? > What is the better disolvent, ethanol or DMSO? The concentration of PMA for oxidative burst depends on the cells youa re using. For example the maximal activation of human PMN can be achieved with 100 nm/ml PMA - but for rats it is abotu 10 ng/ml. The PMA should be in DMSO. I suggest that you make the PMA up in small lots and put it at -20C - it will keep for years that way. Once you thaw it out, toss it out though. Exact details for performing these assays are found in Current Protocols in Cytometry Unit 9.7 which I wrote. Regards Paul Robinson From: "Susana Hernandez Prieto" <shernandez@olemail.com> Organization: hugu To: cyto-inbox Subject: PMA Date sent: Fri, 25 Jun 1999 11:18:32 +0100 > > > Hello flowers: > I´m measuring intracellular H2O2 in leukocyte PMN using H2DCFDA. I would > like to do it in basal conditions and after the activation with PMA > (Phorbol 12-myristate-7-acetate). > Does anyone knows what concentration of PMA has to be used? > What is the better disolvent, ethanol or DMSO? > > Thanks in advance!! > ================================================== > Susana Hernández Prieto. > Unidad de Investigación. > Hospital Universitario Guadalajara > C/ Donante de Sangre s/n > 19002 Guadalajara > Spain > e-mail: shernandez@olemail.com > ================================================== > Tu E-Mail Gratis en http://www.mixmail.com J.Paul Robinson, PhD PH:(765)4940757 Professor of Immunopharmacology Professor of Biomedical Engineering Purdue University FAX:(765)4940517 EMAIL:jpr@flowcyt.cyto.purdue.edu WEB: http://www.cyto.purdue.edu
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