Hello flowers: I´m measuring intracellular H2O2 in leukocyte PMN using H2DCFDA. I would like to do it in basal conditions and after the activation with PMA (Phorbol 12-myristate-7-acetate). Does anyone knows what concentration of PMA has to be used? What is the better disolvent, ethanol or DMSO? Thanks in advance!! ================================================== Susana Hernández Prieto. Unidad de Investigación. Hospital Universitario Guadalajara C/ Donante de Sangre s/n 19002 Guadalajara Spain e-mail: shernandez@olemail.com ================================================== Tu E-Mail Gratis en http://www.mixmail.com
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