--- On Fri, 18 Jun 1999 14:29:26 -0700 (PDT) Maciej Simm <simmmmer@yahoo.com> wrote: >We're experiencing consistnat difficulties w/lysing of RBC in >lymphocyte immunoaphenotyping. Our diff. is in the "noise" around >the lymphocyte population, so that we can't do significant numerical >analysis. The problem is usually conicident with young patients who >are receiving a lot blood transfusions (such as THAL patients). We >think that their RBC's are too strong for our lysis protocol. But at >the same time we need to preserve the lymphocyte cell membranes b/c >we're staining for a variety of CD markers. If anyone has any insight >in this are please don't hesitate to share ;) You can add CD45 labeling to your panel and use this as a marker to identify the lymphs from the rbcs. Gate on CD45+ then look at your other markers. Tom -------------------------------------------------------- Thomas W. Mc Closkey, Ph. D. Director, Flow Cytometry North Shore University Hospital Biomedical Research Center 350 Community Drive Manhasset, Long Island, New York 11030 ph: 516-562-4844 [office]; 516-562-1135/4641 [lab] fax: 516-562-2866 6/23/99 10:12:10 AM E-mail: thomasm@nshs.edu --------------------------------------------------------
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