Hello, We're experiencing consistnat difficulties w/lysing of RBC in lymphocyte immunoaphenotyping. Our diff. is in the "noise" around the lymphocyte population, so that we can't do significant numerical analysis. The problem is usually conicident with young patients who are receiving a lot blood transfusions (such as THAL patients). We think that their RBC's are too strong for our lysis protocol. But at the same time we need to preserve the lymphocyte cell membranes b/c we're staining for a variety of CD markers. If anyone has any insight in this are please don't hesitate to share ;) Maciej Simm Research Technician Weill Medical College of Cornell University _________________________________________________________ Do You Yahoo!? Get your free @yahoo.com address at http://mail.yahoo.com
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