Here is my summary of opinions from round the cytometry group: thanks folks! PS. these are only opinions I recieved: my own views are not included! Approximately 70% of responders favoured FACScaliber. The rest preferred Coulter, apart from one responder who used a MoFlo and liked it very much. FACS pros: Negative population is shown as a discreet population More 4-colour reagents are available Machine and computer are faster in use. Less of a learning curve in upgrading from FACscan Easier to use Good technical backup Upgradable to sorting and dual laser FACS cons: More expensive Coulter XL pros: Well developed data acquisition system Superior fluidics(sample acquisition can be halted without wasting sample) Gating can be altered during acquisition Good technical and service support Good interface for clinical samples Cheaper than FACS Coulter cons: No upgrade to sorting Not as good with dim fluorescence Negative population not displayed -leading to difficulty in setting compensation MoFlo pros: Speed of sorting (c. 20-40,000 cells/sec) Simple design Easy adjustments MoFlo cons: Smaller user base No UK office No local knowledge of machine
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