Chuck Radford wrote: >Does anyone out there know how to calculate the ratio >of fluorescence to FALS of each cell and collect it >as a histogram using CellQuest? I know that Cicero >software is capable of doing that, but I'm not sure >if CellQuest can do that. This is not possible with CellQuest. However, it should be possible with each of these Macintosh programs: FlowJo <http://www.treestar.com/> or FCS Assistant <http://facsmac.med.cam.ac.uk/fcsa.html>. CALC4 for VAX/VMS by Bob Murphy was a nice program for this kind of application, but it only works with LACEL and FCS1.0 files. Eric /\/\/\_ Eric Van Buren, aa9080@wayne.edu \ \ \ Karmanos Cancer Institute and Immunology & Microbiology \_^_/ Wayne State University, Detroit, Michigan, USA
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