Re: CellQuest Question

From: Ray Hicks (rh208@cus.cam.ac.uk)
Date: Sun Jun 20 1999 - 14:26:43 EST


Hi Chuck,

B-D use hardware ratioing on their sorters, and as far as I'm aware, don't
provide an easy way to ratio on their analysers.  If you want to add a
ratio to some already acquired data you can try my shareware FCS Assistant,
available from http://facsmac.med.cam.ac.uk it allows you to add a ratio
parameter to a file for further analysis in cellquest, it also allows you
to export kinetic data to excel etc. for plotting.


Ray

At 12:45 pm -0400 17/6/99, Chuck Radford wrote:
>Does anyone out there know how to calculate the ratio
>of fluorescence to FALS of each cell and collect it
>as a histogram using CellQuest?  I know that Cicero
>software is capable of doing that, but I'm not sure
>if CellQuest can do that.
>
>Thanks.
>
>Chuck Radford
>Brown Cancer Center
>University of Louisville


                              Ray Hicks
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