Hi Chuck, B-D use hardware ratioing on their sorters, and as far as I'm aware, don't provide an easy way to ratio on their analysers. If you want to add a ratio to some already acquired data you can try my shareware FCS Assistant, available from http://facsmac.med.cam.ac.uk it allows you to add a ratio parameter to a file for further analysis in cellquest, it also allows you to export kinetic data to excel etc. for plotting. Ray At 12:45 pm -0400 17/6/99, Chuck Radford wrote: >Does anyone out there know how to calculate the ratio >of fluorescence to FALS of each cell and collect it >as a histogram using CellQuest? I know that Cicero >software is capable of doing that, but I'm not sure >if CellQuest can do that. > >Thanks. > >Chuck Radford >Brown Cancer Center >University of Louisville Ray Hicks ________________________________________________________________________ |University of Cambridge |Tel 01223 330149 | |Department of Medicine |Fax 01223 336846 | |Level 5, Addenbrookes Hospital |e-mail <rh208@cus.cam.ac.uk> | |Hills Road Cambridge |Web http://facsmac.med.cam.ac.uk | |CB2 |ftp server ftp://131.111.80.78 | |UK | | |_________________________________|_____________________________________|
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:38 EST