Dear Flowers In order to induce apoptosis of MDA-MB-468, a breast cancer line, I incubated the cells with 1% FBS/Leibovitz L-15 for 24, 48 and even 72 hours. Then I mounted the cells with a DAPI mounting solution and tried to see some changes in nuclear morphology under fluorescence microscope. To my surprise, I could not see any changes in nuclei between control and tests. My questions, i) is DAPI a good marker for detecting nuclear morphology during apoptosis? ii) do I need RNase in DAPI mounting solution? iii) is the 1% FBS/medium a good inducer of apoptosis? Please give me some advice. Paul Nuclear Medicine Toronto Hospital Toronto, Canada pchen@connection.com
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