We were doing some experiments a few years back where we used trypsin to strip the cell surface so we could stain exclusively for intracellular markers. It was very effective in removing surface markers (we were specifically looking at GPI-linked proteins) and in fact even chewed up some of the intracellular CD3 we were looking at. When I've needed to use the adherent monocytes/macrophages, we've always just scraped them off the flask. Hope this helps! _____________________________________________________ Paula Lavery Transplant Immunology Laboratory McGill University Health Centre Royal Victoria Hospital Montreal, Quebec, Canada plaver@po-box.mcgill.ca "If we knew what we were doing it wouldn't be called research, would it?" -- Albert Einstein -----Original Message----- From: Slava Epelman <sepelman@ucalgary.ca> To: cyto-inbox Date: Thursday, June 17, 1999 2:41 PM Subject: Re: CD14 and trypsin >Hi, > I was wondering if anyone has used trypsin to remove adherent >monocytes/macrophages and then looked for CD14 expression? >What I want to know is if I use trypsin, will CD14 still be reliable monocyte >marker or is it cleaved? >Also, does trypsin alter CD3, CD19 or CD56 expression? >Thanks > >Slava Epelman >sepelman@ucalgary.ca >University of Calgary > > >
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