Nadia As far as I am aware the Macrosort head is fitted as part of the turbosort option on the Vantage (or VantageSE) as well as for sorting large particles, as Brian says you should be OK on a "normal" Vantage with a "normal" head etc. You will need pulse processing if you are sorting your cells for G2/M DNA content (to remove doublets). Ian Ian Titley PhD Leukaemia Research Fund Centre at the Institute of Cancer Research 237 Fulham Road LONDON SW3 6JB UK Tel: +44 171 352 8133 ext5134 Fax: +44 171 352 3299 E-mail: iant@icr.ac.uk > On Wed, 16 Jun 1999 17:26:59 +0200 NADIA.MASTROIANNI.NM@bayer-ag.de wrote: > > > > I have a very naive question: > I'm in the process of starting a new project using Jurkat cells and I would > sort them using a BD FACSVantage. > I've just read an article and in the experimental procedure they write that the > flow cytometer was equipped with pulse processing and MacroSort flow cell. Is > it really necessary to sort Jurkat cells? I thought that MacroSort was useful > for sorting plant cells or pancreatic islets! > > Has someone experience in sorting Jurkat cells? Any particular advice? > > Nadia
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