To Elaine Kunze Sorry about posting this to the list, I tried to e-mail Elaine directly but got an 'undeliverable' message. I am interested in the assay you mentioned for detecting immune cells in milk - do you have a reference to this. I am interested in detecting lymphocytes and antibody secreting cells in bovine milk. Dawn Willix-Payne AgResearch Ruakura Private Bag 3123 Hamilton New Zealand > > > ______________________________ Reply Separator > _________________________________ > Subject: XL question > Author: mek4@psu.edu at INTERNET > Date: 11/06/1999 03:32 > > > I have an XL question. I'm running Doug Redelman's assay for > detecting > immune cells in milk which uses Sybr 1 and PI. They're bright, easily > detectable and so on. Unfortunately, unlike the sperm cell viability > assay, the red and green are mutually exclusive. So if you use red for a > trigger you miss the greens and if you use green you miss the reds. Using > FALS things run fine and you can see the green and redd, but milk > dominates the world. Doug's Partek allows two triggers. Are there any > similar tricks for the XL?? > > > ************************************************************************** > ** > Elaine Kunze > Flow Cytometry.....Image Analysis... > The Biotechnology Institute for Research and Education > Life Sciences Consortium > 8B Althouse Laboratory (814-863-2762) > Penn State University
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