Thanks for all your help. The suggestion to trigger on FL2 is such good one and so obvious after someone else thinks of it. I cant wait to try that today. Also the new XL System II version 3 software (which I just received Friday) has a LISTGATE- function which collects only the particles NOT in the selected gate. This worked well on Friday and is a software alternative to the FL2 trigger. This version has a number of other nice features (coloring Flowset and Flowcom differently, QC templates) so be sure to pester your salespersons if you have an XL and haven't received a copy. **************************************************************************** Elaine Kunze Flow Cytometry.....Image Analysis... The Biotechnology Institute for Research and Education Life Sciences Consortium 8B Althouse Laboratory (814-863-2762) Penn State University
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