XL question

From: Elaine Kunze (mek4@psu.edu)
Date: Thu Jun 10 1999 - 09:12:42 EST


	I have an XL question.  I'm running Doug Redelman's assay for detecting
immune cells in milk which uses Sybr 1 and PI.  They're bright, easily
detectable and so on.  Unfortunately, unlike the sperm cell viability
assay, the red and green are mutually exclusive.  So if you use red for a
trigger you miss the greens and if you use green you miss the reds.  Using
FALS  things run fine and you can see the green and redd, but milk
dominates the world.  Doug's Partek allows two triggers.  Are there any
similar tricks for the XL??


****************************************************************************
	Elaine Kunze
	Flow Cytometry.....Image Analysis...
	The Biotechnology Institute for Research and Education
	Life Sciences Consortium
	8B Althouse Laboratory  (814-863-2762)
	Penn State University



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