Hi Elaine
Apart from removing the milk, I assume both dyes should have
a considerable overlap into the orange FL2 channel, so you
should be fine in using that. Alternatively you can add an
independent red marker onto the cells to make them red and
green. I don't know if a red fluorescent cd45 would work in
milk.
Regards
Gerhard
______________________________ Reply Separator _________________________________
Subject: XL question
Author: mek4@psu.edu at INTERNET
Date: 11/06/1999 03:32
I have an XL question. I'm running Doug Redelman's assay for detecting
immune cells in milk which uses Sybr 1 and PI. They're bright, easily
detectable and so on. Unfortunately, unlike the sperm cell viability
assay, the red and green are mutually exclusive. So if you use red for a
trigger you miss the greens and if you use green you miss the reds. Using
FALS things run fine and you can see the green and redd, but milk
dominates the world. Doug's Partek allows two triggers. Are there any
similar tricks for the XL??
****************************************************************************
Elaine Kunze
Flow Cytometry.....Image Analysis...
The Biotechnology Institute for Research and Education
Life Sciences Consortium
8B Althouse Laboratory (814-863-2762)
Penn State University
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:36 EST