Hi Ragip, There are quite a few people around who analyse bacteria and somewhat less, who also sort them. They use all kinds of machines and protocols. I have been working with Flow-Cytometry and bacteria for a few months now. The instrument is a MoFlo (Cytomation, Colorado) equipped with two water cooled argon lasers and high speed sorting capability. The stains I have used so far are mostly DAPI (requires UV excitation) and SYTO13 (Molecular Probes). A crucial point may be to use fluorescence as a trigger signal instead of side scatter (which is low) or even forward scatter (which is almost non-existent in my current setup). Although or bacs from natural aquatic samples are very small (frequently down to 0.2micron) I haven't found any detection limit so far. Analyses were compared to counts done by fluorescence microscopy. Furthermore, we checked sorted bacteria in the microscope. To my impression, the flow-cytometer detects even smaller cells than you would see in the microscope, but that has not been thouroughly checked yet. good luck Stefan -- Stefan Andreatta University of Innsbruck, Institute of Zoology and Limnology Technikerstrasse 25, A-6020 Innsbruck, Austria phone:++43-512-507-6122; fax:++43-512-507-2930 http://zoology.uibk.ac.at/limno/ ------------------------------------------------------------------------------- Ragip Unal wrote: > > Does anyone know any flow cytometric metod which can detect and sort > bacteria ? If there is waht is the detection limit of detecting bacteria ? > All your will be greatly appreciated. > > Ragip Unal > Food Microbiology and Safety Laboratory > The Ohio State University > Department of Food Science and Technology > 122 Vivian Hall, 2121 Fyffe Rd. > Columbus, OH 43210
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