----->>> On 10-Jun-1999, Ng Bee Ling wrote: > I am looking for a software so as to determine the % of cell cycle > phases (with gating properties) from listmode files. Is there such > software that could run both for files collectd from EXPO and > Cellquest? -------------- FlowJo in an offline analysis package that will read both EXPO (PC) and CellQuest (Mac) files. You can compute the percentages in each of the phases (as well as under-G1 and over-G2) for any gated population. We currently have a limitation in the ability to export all of the cell cycle statistics for all of the samples to a spreadsheet in a single step, but that is implemented in the next release. More info at: http://www.treestar.com/flowjo/html/cellcycle.html I get a lot of requests for the references to the models, so let me give them here: 1) Watson Pragmatic Cytometry 8:1-8 (1987) Watson, Chambers, & Smith: A Pragmatic Approach to the Analysis of DNA Histograms with a Definable G1 Peak 2) Dean Jett Fox Cytometry 1:71-80 (1980) Fox: A Model for the Computer Analysis of Synchronous DNA Distributions Obtained by Flow Cytometry 3) Two Populations Uses Dean-Jett model (with Fox modification) for both populations. Adam ----------------------------- Adam Treister adam@treestar.com http://www.treestar.com/flowjo 800-366-6045 -----------------------------
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