We have a group that is trying to detect antibody staining of antigens on the surface of gram-positive bacteria. Does anyone have experience with this? (Or, more generally, analysis of gram+, or of surface antibody staining on gram-negatives). We'd be interested in seeing some typical methods (what colors do you use, do you use second step reagents) and to see some typical profiles of both scatter and fluorescence. Thanks for any information! mr
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