Khalid Al-Hussein wrote: > Hello Flowers, > I have a graduate student who is interested in counting and > determining the viability of bacteria (pesudomonas. sp, cultured in > different pH concentrations) using Flow. > > Can anyone suggest how we could do this?. We recently wrote a chapter in Current Protocols on viability determinations in bacteria: Davey, H.M., Kaprelyants, A.S., Weichart, D.H., and Kell, D.B. (1999). Approaches to the estimation of microbial viability using flow cytometry. In Current Protocols in Cytometry: 11. Microbial Cytometry, pp 3.1-3.20. New York: Wiley. and there are many other papers on the subject. For counting you will either need a flow cytometer that performs accurate, absolute counts or you can spike your samples with beads of a known concentration. Haze ---------------------------------------------------------- | Hazel Davey hlr@aber.ac.uk | |Sefydliad y Gwyddorau Biolegol*Inst. Biological Sciences| |Prifysgol Cymru * University of Wales| | ABERYSTWYTH, Ceredigion, CYMRU / WALES SY23 3DD | | http://pcfcij.dbs.aber.ac.uk/index.htm |
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