See also the microbiology section on the Purdue CD rom
http://www.cyto.purdue.edu/flowcyt/research/micrflow/gerhard
/gerhard.htm
which contains a section on counting.
Aeruginosa wildtypes are masters in dye exclusion, so be
prepared that a number of them needs a lot of dye. Together
with their low light scatter signal as mentioned by Howard
that can be a potential problem.
Expect discrepancies between membrane integrity and
culturability with pH stress.
Good luck
Gerhard
______________________________ Reply Separator _________________________________
Subject: Counting bacteria?
Author: khussein@smtpgw.kfshrc.edu.sa at INTERNET
Date: 17/05/1999 04:31
Hello Flowers,
I have a graduate student who is interested in counting and
determining the viability of bacteria (pesudomonas. sp, cultured in
different pH concentrations) using Flow.
Can anyone suggest how we could do this?.
Thanks for your time and help.
Dr. Al-Hussein
Head, Flow Cytometry Core Facility
Biological and Medical Research
King Faisal Specialist Hospital &
Research Center
Kingdom of Saudi Arabia
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