Using "Fc-block" (Pharmingen), the monoclonal antibody 24G2, should block a significant amount of this background. Also, make sure the reagent does not have aggregates by spinning in a microfuge (5000xg for 5 min.). The choice of lasers depends on what you want to do - so what applications do you want to support ? Good luck, Rachel ****************** Rachel M. Gerstein, Ph.D. Asst. Professor Dept. of Mol. Gen. & Micro. University Of Massachusetts Medical School 55 Lake Avenue North Worcester, MA 01655 Joseph Webster wrote: > Hi All, > One group here can't use PE-Cy5 dyes because they bind to the B cells > of NOD mice. > PE alone works fine, so the problem is either Cy5 or the combination; > > Does anyone know yet just what the problem is? > > Can anyone tell us if APC-Cy7 or other tandem dyes (or Cy7) have the > same problem? > > Another question: > Our current lasers are argon (488) and a dye (~600). > I am (wishfully) thinking of going to a 3-laser setup with argon > (488), HeNe (635) and a spectrum laser for choice of UV or other lines. > Any helpful comments &/or suggestions? > > Many thanks, Joseph. > > Joseph Webster, Flow Cytometry Facility, > Centenary Institute > Locked Bag No.6, Newtown, NSW 2042, AUSTRALIA. > Ph: 61-2-9565-6110 Fax: 61-2-9565-6101 > e-mail: J.Webster@centenary.usyd.edu.au
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