Hi All, One group here can't use PE-Cy5 dyes because they bind to the B cells of NOD mice. PE alone works fine, so the problem is either Cy5 or the combination; Does anyone know yet just what the problem is? Can anyone tell us if APC-Cy7 or other tandem dyes (or Cy7) have the same problem? Another question: Our current lasers are argon (488) and a dye (~600). I am (wishfully) thinking of going to a 3-laser setup with argon (488), HeNe (635) and a spectrum laser for choice of UV or other lines. Any helpful comments &/or suggestions? Many thanks, Joseph. Joseph Webster, Flow Cytometry Facility, Centenary Institute Locked Bag No.6, Newtown, NSW 2042, AUSTRALIA. Ph: 61-2-9565-6110 Fax: 61-2-9565-6101 e-mail: J.Webster@centenary.usyd.edu.au
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