>Has anyone ever used protein attached to latex beads as a target for >antibody evaluation by flow? I have a 19 KDa recombinant protein for >Plasmodium falciparum that I want to use as a probe to look for IgG >subclasses in human sera. Any suggestion (comercial source, protocol for >protein attachement, any trick?????) Olindo, I have used 5 micron sulfated latex beads from Interfacial Dynamics Corp. (product number 1-5000; ph 800-323-4810) as artificial APCs by coating them with antibodies [proteins]. You just add your protein (in PBS @ ~10µg/ml) to the beads (~10^7) and incubate for ~2 hours and block. Such beads when coated w/ protein can be used as targets for antibodies and their light scatter and fluorescence properties can easily be assessed by flow cytometry. AW Andrew D. Wells, Ph.D. University of Pennsylvania Department of Medicine 904 Stellar-Chance Laboratories 422 Curie Boulevard Philadelphia, PA 19104 (215) 898-1951 (215) 573-2880 (FAX) adwells@mail.med.upenn.edu
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