Hello everyone, I have just started a new project measuring the change in intracellular calcium in neutrophils using flow cytometry. No one in our lab has done this before so I just started using fluo-3 because we had some. In my readings I am lead to believe that a ratiometric approach using fluo-3/fura red is a better way to go. I have done a literature search and have only found one reference by Novak and Rabinovitch in 1994 describing the method. I would like to know: 1. Which method people are currently using for a bench top analyzer with a 488nm argon laser? 2. Is anyone really using the fluo-3/fura red method? 3. Is this method better than just using fluo-3 alone? Your input will be greatly appreciated.
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