We are currently evaluating the instrument in our lab, and simultaneously analyzing CD34 using ISHAGE guidelines, ProCount and the IMAGIN2000. The samples of primary interest are peripheral blood and PBPC collection products from apheresis stem cell collections. Thus far the peripheral blood has had excellent correlation with all 3 methods in both the low (~10 per uL) and high (200-700 CD34 per uL) ranges of CD34. The PBPC samples require dilution for both the ProCount and IMAGIN, which we are attempting to standardize relative to nucleated cell concentration and CD34 frequency. We are predominately interested in the peripheral blood as a STAT method in determining when a donor/patient CD34 level is optimal for collection. A secondary feature would be in determining the CD34 dose in the collection product, to determine whether a second collection is required, therefore whether cytokine stimulation must be continued. April Translational Research & Flow Cytometry Blood & Marrow Transplantation Univ TX MD Anderson Cancer Center
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