Patrica- I am assuming, from what B-D suggested, that you will use the PharRed (Cy7APC is the generic name) with APC. Then, depending on which PMT position you will be using for collecting Cy7APC, you will need either a short pass or long pass beam splitter at 710 nm. The bandpass filter 780/60 will work well for Cy7APC. When you specify the beam splitter, you need to mention that it will be mounted at a 45 degree angle. You may need to purchase a beam splitter holder from BD if you don't have an extra or one you can scavange. We have ordered filters from Chroma Tecnology, also in Vermont. Their phone # is 800-824-7662. Similar filters that we have ordered have cost from $200 to $300 each. For best results using Cy7APC it is necessary to have a red sensitive PMT. The Hamamatsu R3896 have worked well for us. I do not know what B-D supplies in their instrument. Good luck! -Marty Bigos Stanford Shared FACS Facility
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