Re: Pharmingen SAV-Phar Red antibody

From: Larry Arnold (lwarma@med.unc.edu)
Date: Fri Apr 30 1999 - 17:41:16 EST


Patricia

We do PharRed on our MoFlo.  Got the filters from Omega.  About $150.

Larry

At 05:08 PM 4/29/99 -0500, you wrote:
>
>	Greetings!
>
>	I have a problem that perhaps someone can help me with:
>
>	I am trying to set up some 4 color panels on my VantageSE. (I have
>the UV-488 and HeNe). I purchased, based on a chart in the Pharmingen
>catalog which indicated it was appropriate for the Vantage, and a
>recommendation from Pharmingen, a SAV-PharRed to use with my biotinylated
>antibodies.
>
>	I found out this morning that I need a filter set from BD to see
>this, but since it is SO new, there is no price associated with it. B-D
>decided to  "quote high"......(translation, $1500). So, in other words, I
>shouldn't have  bought this flurochrome unless I knew I could read it....my
>mistake....hope not to make that one again!
>
>	But maybe someone out there will know where a I can get a filter
>set ..or just a filter that will allow me use tis flurochrome!?!?!). It
>almost looks like I am the first person to ask about this. Can I be so
>lucky?  :-D
>
>	B-D was helpful in telling me that Omega may be the company who
>makes them, and gave me their phone number (of course, it was 5:30, and they
>closed at 5:00).
>
>	Can anyone tell me any other company that might make the same thing,
>OR if only one of the two filters will work on this machine? ALso, this
>flurochrome excites at 633 and emits (peak) at 780. Here they are:
>
>	DM mirror 710
>	BP DM 780/60
>
>	It seems that the BP 780/60 alone would work ($450 if I'm
>lucky)....hope someone can help!
>
>	TIA,
>
>	Patricia Echeagaray
>*****************************************************************
>Patricia L. Echeagaray
>Flow Cytometry Services
>Serquest.....a Southern Research Institute Company
>431 Aviation Way
>Frederick, MD 21701
>          USA
>Voice:  301-694-3232 ext 276
>FAX:  301-694-7223
>echeagaray@serquest.com
>*****************************************************************
> 



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