Dear Sasha, I forgot an important point - we have 4 PMT detectors on laser 1, and have measured PE-Cy7 off the 1st laser as well as APC-Cy7 off the 2nd laser. We use the same 780/60 BP filter for both tandem conjugates involving Cy7 and the 710 LP dichroic as well. I have not tested a 710 SP dichroic for Cy7 reagents. You ought to use a BP in addition to your mirror to minimize leakage from other areas of the spectrum. We have left the standard 610 SP in place to reflect signal to the 710LP dichroic. Sorry for the omission, Joe
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