Claudio: Protocols for PE and Cy5PE conjugation can be found on my web site (www.drmr.com/abcon/). For PE, if you don't want to work the whole thing up yourself, you can purchase kits from ProZyme (www.prozyme.com). These kits let you conjugate 1 mg of antibody (per kit) to PE; it takes about 2 hours from start to finish, and the kit comes with everything you need. mr
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