Hi everybody, we would like to try to make our own Ab-fluorochrome conjugates. In our hands this already works for FITC, but we would like to do this also with fluorochromes which are detectable in Fl-2 and Fl-3. (Emission at 585/42, respectively 650 LP). The fluorochromes should be excitable with 488 nm. We already know a protocol for PE conjugation, though we are not satisfied with the results when comparing to commercially available Ab conjugates. Does anybody know about some kits (perhaps with other fluorochromes) which could help us further? Any help is greatly appreciated Claudio Vallan =================================================== Claudio Vallan PhD Phone Lab: 031 / 632 88 76 FACS-LAB DKF Phone Office: 031 / 632 99 68 University of Bern E-Mail: vallan@dkf7.unibe.ch c/o Institute of Pathology Murtenstrasse 31 Insel hosptial area only: 3010 Bern Beeper: 181 67 59 ===================================================
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:23 EST