I found lots of useful information regarding antibody conjugation at the following website: http://www.drmr.com/abcon/ Paula Lavery Transplant Immunology Laboratory Royal Victoria Hospital McGill University "If we knew what we were doing it wouldn't be called research, would it?" -- Albert Einstein -----Original Message----- From: Claudio Vallan <claudio.vallan@dkf7.unibe.ch> To: cyto-inbox Date: Tuesday, April 20, 1999 2:30 PM Subject: Fluorochrome conjugation to Ab > > Hi everybody, > >we would like to try to make our own Ab-fluorochrome conjugates. In our >hands this already works for FITC, but we would like to do this also with >fluorochromes which are detectable in Fl-2 and Fl-3. (Emission at 585/42, >respectively 650 LP). The fluorochromes should be excitable with 488 nm. We >already know a protocol for PE conjugation, though we are not satisfied >with the results when comparing to commercially available Ab conjugates. >Does anybody know about some kits (perhaps with other fluorochromes) which >could help us further? > >Any help is greatly appreciated > >Claudio Vallan > >=================================================== >Claudio Vallan PhD Phone Lab: 031 / 632 88 76 >FACS-LAB DKF Phone Office: 031 / 632 99 68 >University of Bern E-Mail: vallan@dkf7.unibe.ch >c/o Institute of Pathology >Murtenstrasse 31 Insel hosptial area only: >3010 Bern Beeper: 181 67 59 >=================================================== > > >
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