Dear James,
WinMDI Version 2.8 allows you to combines graphical regions in various
ways using
Boolean logic. If R1 is a region in a dotplot of FCS vs SSC, for example,
and R2 is another
region drawn over PI + (dead) cells in a histogram, then you could use a
"gate" where
the logic is AND for R1 and NOT for R2 to select cells in R1 and cells not
in
R2 as the ones to plot in another window. This is done using the Logical
Gate Tool.
For more specific information feel free to contact me directly with your
questions on how
you might gate your data set. If you are wanting to analyze BrdU FITC +
subsets, then
a glimpse of your actual data would be helpful in pointing you in the right
direction.
WinMDI 2.8 is not unique in its method of using logical gates. CellQuest
and other
flow applications work in a very similar way.
Joe
Joseph Trotter
Director, Flow Cytometry
The Scripps Research Institute
10550 North Torrey Pines Road
La Jolla, California
----- Original Message -----
From: James Liou <jliou@bu.edu>
To: cyto-inbox
Sent: Tuesday, April 06, 1999 2:46 PM
Subject: WinMDI gates; BrDu protocol
>
>
> Dear colleagues,
>
> 1) Does anyone know how to gate using the WinMDI program? The
help
> section was not very helpful and the logical gating system wasn't too
logical
> to me. I'd appreciate some help please.
>
> 2) Could someone provide a protocol/reference for BrDu labeling
and
> staining for DNA synthesis analysis by FACSCAN? Preferably not a
reference in
> Cytometry because we don't get it in our library.
>
> Thanks in advance!
>
> Sincerely,
>
> James Liou
>
>
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:21 EST