I can't help you with the first question, but I ca with the second. Pharmingen and Phoenix FLow Systems both sell a kit that will measure this. The Phoenix system comes with directions which also show you how to set up the FACScan for analysis. P. Echeagaray > -----Original Message----- > From: James Liou [SMTP:jliou@bu.edu] > Sent: Tuesday, April 06, 1999 5:47 PM > To: Cytometry Mailing List > Subject: WinMDI gates; BrDu protocol > > > > Dear colleagues, > > 1) Does anyone know how to gate using the WinMDI program? The > help > section was not very helpful and the logical gating system wasn't too > logical > to me. I'd appreciate some help please. > > 2) Could someone provide a protocol/reference for BrDu labeling > and > staining for DNA synthesis analysis by FACSCAN? Preferably not a > reference in > Cytometry because we don't get it in our library. > > Thanks in advance! > > Sincerely, > > James Liou >
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