Has anyone out there used a Becton Dickinson FACScan or FACSort flow
cytometer to analyze platelet- or WBC-derived microparticles (0.2-1.0 um in
size)? I am looking for assistance in the following arenas:
1. Distinguishing background (noise) events from "real" microparticle
events; looking for ways to increase the limit of sensitivity of
these instruments (Becton Dickinson Suggests limit is 0.5 to 1.0 um).
2. Setting used during acquisition; especially, threshold trigger
(FSC or FL) and values thereof
3. Gating strategies
4. Sample collection and staining
Please respond to laurie_stojanovic@baxter.com with any information you
have that could help me. I may also be reached at (847)270-4422.
Thanks!
Laurie Stojanovic
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