Girakhoo, Soheila wrote: > Hi > Could somebody suggest me about apoptosis satining with Annexin V-FITC and PI > for Flow Cytometry ? > I want to stimuli my cells befor staining with DEX, etopside, Staurosporine, > Actinomycin D. > Thanks, Soheila > There's plenty of literature available on this application . . . do a search on annexin V and flow cytometry. This will result in a wealth of info that will include your apoptosis inducers. As a beginning, I usually start with the following:Darzynkiewicz Z, et al. Cytometry in Cell Necrobiology: Analysis of Apoptosis and Accidental Cell Death (Necrosis). Cytometry 27: 1-20 (1997) You can also check with reagent suppliers (PharMingen, Phoenix Flow Systems . . . Coulter and Caltag, too, I think . . .). Their reagents will come with detailed procedures for labeling. -- Mark A. KuKuruga, Managing Director University of Michigan Core Flow Cytometry kukuruga@medmail.med.umich.edu
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:20 EST