Derek Davies wrote: > Hi Laura, > You are right in saying that the acid treatment destroys surface staining so > the DNAse method is the one to go for. . . . There is precedent that a biotin-conjugate surface antibody will survive the acid denaturation step necessary for BrdU detection.However, remember the recently (perhaps not-too-recent . . .) reported strand break induced photolysis method. Look at Phoenix's web site <http://www.phnxflow.com/> for a good explanation of this with their Absolute-S kit. This method reveals incorporated BrdU without the denaturing effects of acid or heat, so preserves surface antigens well. MAK. -- Mark A. KuKuruga, Managing Director University of Michigan Core Flow Cytometry kukuruga@medmail.med.umich.edu
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