I would also be interested in any responses to this question, so please post to the list. Thank you, Antony Bakke, PhD Oregon Health Sci Univ Dept of Pathology bakkea@ohsu.edu >>> Rohan and Shanthi Ameratunga <immunology@xtra.co.nz> 03/30 8:20 PM >>> Dear Colleagues, I would be grateful for advice on CTLA4 staining. We are attempting to stain cell surface CTLA4 as marker of solid organ rejection in peripheral blood lymphocytes. None of liver or kidney transplant samples have been positive using the Coulter immunotech antibody. We are not sure if this is a technical problem or whether the antigen is not expressed in the periphery. 1. What in vitro stimulation methods should be used for positive controls? How long should cells be stimulated before we detect CTLA4? 2. How useful is intracellular staining for this antigen? Thank you in advance Best regards Rohan Ameratunga Auckland Hospital NEW ZEALAND
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