subtraction program immuno-4

From: Mark Cozens (cozens@postoffice.utas.edu.au)
Date: Tue Mar 23 1999 - 18:00:56 EST


Dear Flow-ers

		I'm a student using Mark's email (Kristy Weir).
I'm using Immuno-4 subtraction program on a coulter elite.  Is overton
subtraction/Immuno-4 used widely? How acceptable is it for publishing data?
I'm looking at sheep dendritic cells for phenotype analysis.  Negative
controls are within the first log decade and are quite clean. I've compared
Immuno-4 to placing a gate manually and the results are quite comparable
(within 2-5% difference).
Any help would be much appreciated, thanks in advance.

Mark A Cozens
Division of Pathology
University of Tasmania
ph	03 62264828
fax	03 62264833



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