Dear Flow-ers I'm a student using Mark's email (Kristy Weir). I'm using Immuno-4 subtraction program on a coulter elite. Is overton subtraction/Immuno-4 used widely? How acceptable is it for publishing data? I'm looking at sheep dendritic cells for phenotype analysis. Negative controls are within the first log decade and are quite clean. I've compared Immuno-4 to placing a gate manually and the results are quite comparable (within 2-5% difference). Any help would be much appreciated, thanks in advance. Mark A Cozens Division of Pathology University of Tasmania ph 03 62264828 fax 03 62264833
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