Hello, Flow Users: Bone marrow megakaryocytes, the precursors of platelets, mature via endoreduplication, forming normal populations of 4N-64N cells that become larger as the nucleus expands with DNA. These cells can be identified in bone marrow preparations by gating on FSC (larger size) and FL1 after staining for one of their surface glycoproteins, such as GPIIbIIIa, with a FITC antibody. Once gated, their ploidy content can be measured as a series of DNA peaks by also staining with PI. Kathryn L. Kellar, PhD (Kathi) Biologics Branch SRP/NCID Centers for Disease Control and Prevention 404-639-1419 klk1@cdc.gov
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