Hi CastilloNavarrete and flowers! I have some experience with permeabilization in platelets. I mesured Fibrinogen, vW and GP 53 intraplatelets. At the begining I tested diferent products one is sold by Becton and Dickinson and the other is from Immonotech (Intraprep Cat N 2388). I had good results with both, but actually I am working with the second . I used CD63 with or without permeabilization as control, if you nedd more informtion write me. On the other hand I worked with TO to measure reticulated platelets, I used a dilution 1/8 and 1/4 in PBS without permeabilization but I have a lot of difficults with the expresion of the results, now I am working in it to find a good standarization, if you know something tell me. Thanks and I hope anwers!Emilse EMILSE BERMEJO Instituto de Investigaciones Hematologicas, Academia Nacional de Medicina de BsAs ARGENTINA e-mail <emilse@connmed.com.ar>
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