I don't work with ploidies, etc, but I could throw in an idea. In the lab where I did my Phd there was a group culturing rat uterine stromal cells. They talked about 'endoREduplication'. I vaguely remember that is was like mitosis started but not completed, resulting in strange ploidies. According to my medical dictionary: endomitosis: " ... variable stages of mitosis occur within the nucleus, including chromosome replication (= endoreduplication), but the mitotic apparatus and metaphase plate do not form and the nucleus does not divide. If no obvious mitotic stages occur, then an increase in ploidy will be the only evidence (= masked endomitosis)." Heidi Engelhardt > We too see the unexpected 8N etc. cell ploidies in some of our cell cultures and > haven't had a good explanation for same. The "endoduplication" explanation > seemed popular. Sorry to seem daft and ignorant but I surveyed a number of our > cell culture people and molecular types here and no one could define > endoduplication for me. In fact, no one had heard the term before. The mail > camel has not yet arrived with my latest copy of cytometry so I am at a loss for > an explanation of what endoduplication is. Could someone help this poor soul? > > Thanks > > Haywood Pyle > KFSH, MBC#3 > Box 3354 > Riyadh 11211 > Saudi Arabia > e:pyle@kfshrc.edu.sa > > Heidi Engelhardt Department of Animal and Poultry Science University of Guelph Guelph, Ontario CANADA N1G 2W1 phone: 519-824-4120, ext. 3658 or 8355 fax: 519-767-0573 email: hengel@aps.uoguelph.ca www: http://www.aps.uoguelph.ca/faculty/heidi.html
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