If you get replies that are not posted to the board, could you post them for general information? To my knowledge, CD62L is the most labile antigen, as far as stability. I am not aware of problems with CD3, CD4, or CD8, but then I haven't systematically looked at fluorescence intensity in cells frozen for various periods of time. When they are there before freezing, they are there after. CD38 expression is supposed to be well-preserved with freezing (anecdotal observations from Janis Giorgi). > -----Original Message----- > From: Glenda, M, Davison [SMTP:gmd@samiot.uct.ac.za] > Sent: Thursday, March 04, 1999 12:27 PM > To: Cytometry Mailing List > Subject: antigen expression after freezing > > > Dear All, > > I would really appreciate any advice on this question. We have been > freezing lymphocytes on patients post bone marrow transplantation > since 1995. After thawing one of the samples to perform > CD45Ra and RO analysis I was horrified to find that it had only > +/- 16% viability (using trypan blue). I set it up anyway and was > surprised to get good CD3 and CD45 positivity. CD4 and CD8 did not > give satisfactory results and it was difficult to distinguish between > positive and neagtive peaks. The question is: Are certain antigens > preserved in cell death? I dont have much experience in the > effects of freezing on cells and would therefore appreciate any > advice. > > Thanks, > Glenda Davison > Dept.Haematology > Groote Schuur Hospital > Cape Town South Africa > e-mail:gmd@samiot.uct.ac.za
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