Dear All, I would really appreciate any advice on this question. We have been freezing lymphocytes on patients post bone marrow transplantation since 1995. After thawing one of the samples to perform CD45Ra and RO analysis I was horrified to find that it had only +/- 16% viability (using trypan blue). I set it up anyway and was surprised to get good CD3 and CD45 positivity. CD4 and CD8 did not give satisfactory results and it was difficult to distinguish between positive and neagtive peaks. The question is: Are certain antigens preserved in cell death? I dont have much experience in the effects of freezing on cells and would therefore appreciate any advice. Thanks, Glenda Davison Dept.Haematology Groote Schuur Hospital Cape Town South Africa e-mail:gmd@samiot.uct.ac.za
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